Date Full Report Received09/30/2004
Date Abstract Report Received09/30/2004
InvestigationInstitution: University of Nebraska
Primary Investigator: Dr. Michael Zeece, Dr. Steve Jones
Funded ByIowa Pork Producers Association
With the advent of molecular biology techniques it is possible to examine cellular events that cause problems in the quality of the meat. One such technique is called proteomics. The rationale for using a proteomics approach is it can provide a broad spectrum of information including the profile and assessment of proteins essential to calcium regulation. Abnormal calcium regulation is the root cause for expression of the PSE condition postmortem. This study examined ways in which a large number of muscles could be examined to identify changes in the protein that may be responsible for the calcium regulation. We were able to develop procedures that would rapidly isolate the proteins in question from samples that could be obtained by muscle biopsy. Once the protein could be separated and quantified using procedures a whole host of the proteins could be examined simultaneously. From the results procedures have been developed to determine differences between protein in the ryanodine receptor and another protein, SR Ca+2 APTase, that regulates the calcium level in muscle cells.