Date Full Report Received


Date Abstract Report Received


Primary Investigator:

Objectives: Objective 1: To validate the diagnostic specificity of the currently approved APHIS PCR protocols for detection of CSF, ASF, and FMD in oral fluids.
Objective 2: To validate the diagnostic specificity of non-USDA licensed, Tetracore commercial RT-PCR kits for detection of each disease- CSF, ASF, and FMD in oral fluids.

Materials & Methods:
South Dakota- Animal Disease Research & Diagnostic Laboratory tested samples from districts 3-6. Each of the laboratories tested 255 oral fluid samples for the three diseases of interest by both the APHIS approved NVSL PCR assays and the Tetracore commercial kits.

• District 3: Minnesota, Wisconsin, Michigan, Ohio, Indiana, Iowa, Illinois, Kentucky
• District 4: Missouri, Arkansas, Louisiana, Mississippi, Oklahoma, Texas.
• District 5: Montana, North Dakota, South Dakota, Nebraska, Kansas, Wyoming, Idaho
• District 6: Colorado, New Mexico, Utah, Arizona, Nevada, California, Oregon, Washington