Mechanisms of Failed Protection Against PRRS in Sow Herds

Serum inoculation of gilts with on-farm isolates is used to protect endemically infected sow herds against reproductive PRRS. However, outbreaks still occur in which the offending virus is genetically similar to the inoculation virus across the entire genome, raising the…

Physical Characterization of the PRRSV Virion

The research here shows that PRRSV grows in cells as a population of viruses with the same shape, the same RNA molecules, and the same protein composition. There was no evidence for multiple forms that varied in infectivity or in…

Mechanisms of Failed Protection Against PRRS in Sow Herds

We have established a set of standard protocols and reagents for whole genome PRRSV sequencing and assembly. Phylogenetic comparisons amongst 10 pairs of homologous strains confirmed several regions undergoing positive selection (changes in protein sequences assumed to be in reaction…

Serum Markers of PRRSV Infection

Early detection of infectious disease is crucial for preventing the spread of the infectious agent. In PRRS, current diagnostic methods mainly use the antibody response of individual pigs or herd, which requires at least 7-10 days. Some biological molecules appear…

Direct Physical Characterization of the PRRSV Virion

We have established that PRRSV-infected cells produce only one form (density) of virus and that it accounts for all of the infectivity. The virion is comprised of nucleocapsid at least 5 proteins. Two predicted proteins were not found, and portions…