#06-127

Complete

Date Full Report Received

10/30/2007

Date Abstract Report Received

10/30/2007

Investigation

Institution:
Primary Investigator:

The PRRSV strain database (http://prrsv.ahc.umn.edu) has been supported by the National Pork Board for three years. The database (PRRSVdb; 7,627 unique sequences) is freely available for web-based queries in order to obtain detailed information about PRRSV sequences (percent similarity to vaccines or other database isolates, ORF5 RFLP analysis, year and state of isolation, phylogenetic relationships, GenBank submission numbers). The project, proposed to fulfill the NPB directive to implement a National PRRSV Sequence Database, has relevance to a number of PRRS Initiative efforts such as surveying potentially new field strains for vaccines, examining sequence conservation among various isolates for improvement of diagnostics, epitope evaluation and assessment of virus spread locally, nationally and internationally. The database is comprised presently of 3 major veterinary diagnostic laboratory sequenced isolates (Minnesota Veterinary Diagnostic Laboratory, South Dakota Animal Disease Research &?Diagnostic Laboratory, Manitoba Veterinary Services Branch) and those independently deposited in GenBank. In addition, the appearance of the database has been updated to provide additional flexibility to the PRRSVdb. This new flex-based web interface provides improved phylogeny viewing and user-uploaded sequence analysis, either dependent or independent of the database. Accessing related clinical data will be available through PRRSVdb interaction with other data sources soon. This research is the result of continued interactions of the National Pork Board (Pamela Zaabel), the National Center for Animal Disease (Kay Faaberg) the Minnesota Veterinary Diagnostic Laboratory (MVDL; James Collins and the Molecular Diagnostic Section), South Dakota Animal Disease Research &?Diagnostic Laboratory (Jane Christopher-Hennings, Travis Clement), and the Manitoba Veterinary Services Branch (Andre Hamel) with software engineers (Trevor Wennblom, John Crow and Ernest Retzel) located at the Center for Biomedical Research Informatics (CBRI) of the University of Minnesota.