#18-191 IPPA


Date Full Report Received


Date Abstract Report Received



Primary Investigator:
Co-Investigators: Marcelo Almeida

Processing fluids have been widely used in the US swine industry for PRRSV monitoring in breeding herds. Processing fluids-based testing is an efficient and reliable way to monitor newborn piglets (2-5 days of age). However, the industry still needs a practical and sensitive method to verify the PRRSV status of due-to-wean piglets.
This study evaluated the use of family oral fluids (FOF) sampling for PRRSV detection in due-to-wean piglets, compared to bleeding piglets. FOF is a sample collected by hanging a rope in the farrowing crate where both the sow and respective piglets have access to it. When hung properly, the success rate to obtain the fluids back has been >95%. In other words, you hang 20 ropes and retrieve 19 or 20 FOF. FOF can be submitted to diagnostic laboratories for testing just like a ‘regular’ oral fluid sample (i.e. kept on ice/refrigerated right after collecting).
This study demonstrated that FOF-based monitoring is a practical and efficient strategy to monitor for PRRSV in due-to-wean piglets (15-21 days old) in breeding herds. Using 10, 15, 20, 30 and 40 FOF samples provided an equivalent probability of PRRSV detection by PCR to testing serum samples from 90, 120, 240 and 400 piglets, respectively. When selecting which litters to hang ropes to collect FOF, results from this study suggest that litters from parity 1 sows, and/or litters with fewer number of piglets were more likely to test positive when the virus is present compared to litters from older sows or litters with a high number of piglets.
In summary, FOF is a new, practical, and efficient sample that can be used for PRRSV monitoring in due-to-wean piglets. It is a great tool to be implemented to verify PRRS status in farrowing rooms before weaning time.