#15-208

Complete

Date Full Report Received

02/14/2017

Date Abstract Report Received

02/14/2017

Investigation

Institution:
Primary Investigator:

The overall objective of this research is to evaluate the effectiveness of various chemical mitigation products applied to different feed manufacturing surfaces on the reduction of porcine epidemic diarrhea virus (PEDV) genetic material to mitigate the risk of disease transmission originating from feed manufacturing facilities. The first experiment was to characterize sanitizer’s effectiveness for the surface of materials typically encountered in a feed mill environment. The second experiment was focused on evaluating protocols for reducing PEDV infectivity in the feed manufacturing system where feed contact surfaces are not easily decontaminated using wet cleaning methods. A secondary objective was to evaluate infectivity of dust samples collected during feed manufacturing.

For the first experiment, 5 different feed manufacturing surfaces and 10 sanitizing treatments were evaluated Surfaces included stainless steel, plastic, rubber, woven polypropylene tote bag, and sealed concrete, which were evaluated by applying PED virus, followed by application of the various sanitizing agents. Quantification of detectible genetic material collected via surface swab was then performed using real time, reverse transcriptase polymerase chain reaction (qRT-PCR). Liquid Sal CURB and liquid bleach were the most effective chemical treatments to reduce the quantity of detectable PEDV RNA, but the practicality of their application in field settings is limited due to their liquid state and potential corrosiveness.

For Experiment 2, medium chain fatty acids (MCFA) and formaldehyde were used to treat rice hulls to disinfect laboratory-scale mixers and a production scale mixer and bucket elevator system. Overall, the use of rice hull flushes effectively reduced the quantity of detectable RNA present after mixing a batch of PEDV-positive feed. Chemical treatment of rice hulls with formaldehyde and 10% MCFA provided additional reduction in detectable RNA. Finally, dust collected after manufacturing PEDV-inoculated feed contains a very high quantity of viral RNA and was found infective, demonstrating it has the potential to serve as a vector for PEDV transmission.

Producer bottom line;
Differences in surface structure and porosity affect the ability of chemical disinfection agents to inactivate and remove genetic material
Liquid disinfectants including formaldehyde and sodium hypochlorite (bleach) appear to be efficacious PEDV surface disinfection agents; however, practicality of use and safety in feed manufacturing facilities need to be considered.
Using abrasive agents (i.e. rice hulls) reduces cross contamination and treatment of the rice hulls with formaldehyde or MCFA can further reduce risk for manufacturing feed where wet cleaning methods cannot be easily applied.
Dust can contain a high quantity of PEDV genetic material that can cause infectivity and therefore be a vector for PEDV transfer.
Dr. Jason Woodworth
Kansas State University
Dept. of Animal Sciences & Industry
251 Weber Hall
Manhattan, KS 66506
785-532-1157
jwoodworth@ksu.edu