#16-107

Complete

Date Full Report Received

05/22/2018

Date Abstract Report Received

05/22/2018

Investigation

Institution:
Primary Investigator:

Formerly a “fringe” issue, lameness in swine production has become a priority in the face of recent veterinary and producer-driven lameness investigations showing that losses attributable to lameness can reach $23 per growing pig, and culls or mortality due to lameness can routinely approach 1% to 5% of the total population. The two largest swine diagnostic laboratories in the United States have reported an increase in infectious arthritis due to Mycoplasma hyorhinis and Mycoplasma hyosynoviae. The Iowa State University Veterinary Diagnostic Laboratory (ISU-VDL) diagnosed 890 cases of arthritis between 2003 and 2014. Mycoplasma-associated arthritis accounted for an average of 26% of arthritis cases over that period, with an increasing frequency in recent years.
Despite the common occurrence of M. hyorhinis-associated disease and M. hyosynoviae-associated arthritis, practitioners’ desire improved antemortem diagnostics as well as a better understanding of disease pathogenesis and the immunologic response that can help quantify the effect of potential interventions of Mycoplasma-associated arthritis. Accordingly, the goals of this project were to develop and validate novel diagnostic assays for infectious arthritis due to M. hyorhinis and M. hyosynoviae in growing pigs and to characterize the immunopathology of M. hyorhinis-associated disease. Prior to this project, the number, quality, and quantity of samples necessary to develop reliable antibody ELISA assays were not available to researchers. To address this, our group generated the necessary samples for researchers to develop new tests, developed ELISAs using new technology for the detection of M. hyorhinis and M. hyosynoviae, and established association between detection of either agent in oral fluids by PCR and clinical disease.

Contact information:
Bailey Arruda
Veterinary Diagnostic Laboratory
Iowa State University
1850 Christensen Drive
Ames, IA 50011
wilberts@iastate.edu