Date Full Report Received01/23/2015
Date Abstract Report Received01/23/2015
Funded ByNational Pork Board
The overall objective of this research was to generate biological reagents for detection and characterization of neutralizing antibody to PEDV and Porcine Deltacoronavirus. (PDCoV). The specific goal was to generate luciferase reporter viruses that express the spike (S) protein of PEDV and PDCoV and to use these reagents for detection and quantitation of virus-neutralizing antibody. We were able to generate PEDV virus-like particles but they were found to be poorly infectious in vitro. A variety of cell-culture conditions were tested to improve yield and infectivity of PEDV reporter virus, but we were not able to reproducibly generate high-titered PEDV pseudovirus stocks need for virus neutralization assays. At present, live infectious virus will be needed for analyses of neutralizing antibody in PEDV-infected or suspect pigs.