#03-048

Complete

Date Full Report Received

02/02/2005

Date Abstract Report Received

07/26/2006

Investigation

Institution:
Primary Investigator:
Co-Investigators: F. Chris Minion, James McKean

The use of serological assays to monitor the health status of swine herds has been used for several decades. The primary assays employed have included agglutination, neutralization, and/or ELISA assays to detect the presence of serum antibodies to a specific infectious agent. The current work indicates that a flow cytometric assay can be used to evaluate serum samples for antibodies reactive with multiple antigen in a single reaction tube. The ability to detect antibody responses to five separate Salmonella endotoxin preparations in a single tube was shown. There are several advantages of this type of assay over traditional plate ELISA assays. For example, different set of beads coated with a variety of antigens can be mixed in a single reaction tube. The beads could be coated with bacterial, viral, parasitic, or fungal antigens and combined in a single reaction tube. In addition, the amount of serum sample required for the assay is small (less than 100 µL) regardless of the number of antigen-coated bead sets used in the assay. Lastly, the use of this technology should be useful diagnostically as well as in monitoring the health status of livestock animals.