#05-072

Complete

Date Full Report Received

05/03/2007

Date Abstract Report Received

07/21/2008

Investigation

Institution:
Primary Investigator:
Co-Investigators: Jonathan G. Frye, Siddhartha Thakur

We developed a microarray for the rapid detection and characterization of three important bacterial pathogens including Campylobacter, Salmonella and Yersinia and a viral pathogen including Noroviruses. Probes that were printed on the glass slide were selected after analysis and through testing using control strains. The results from this preliminary study show that we can identify and characterize the pathogen of interest in a very short period of time when compared to the standard laboratory methods used for this purpose. Current standard protocols for the isolation and detection procedures of these pathogens are laborious and have very low sensitivity. In addition, characterizing to specific strain level is time consuming and very costly. Therefore, development of this microarray that is very sensitive, having a low detection limit, time-efficient and a low cost method will enable us to monitor the presence of multiple antimicrobial resistance genes and other important determinants in animals, humans and the environment.

Contact information:
Wondwossen A. Gebreyes, DVM, Ph.D., Dipl. ACVPM
Infectious Diseases Molecular Epidemiology and Detection Laboratory (IDMEDL)
Dept. Vet. Prev. Med., Coll. of Vet. Med., The Ohio State University
A100R Sisson Hall, 1920 Coffey Rd., Columbus, OH 43210
Tel. (614) 292-9559 [Off.], (614) 292-3412 [Lab.], (614) 292-4142 [fax]
Email: Gebreyes.1@osu.edu