Date Full Report Received

04/02/2014

Date Abstract Report Received

04/02/2014

Investigation

Institution:
Primary Investigator:

Since the introduction of porcine epidemic diarrhea virus (PEDV) in the Unites States, millions of piglets have died. Key items are needed to be address to further initial PEDV research, such as development of PEDV propagation techniques, creating PEDV reference samples to share to the research community, and having a reliable PCR test to aid in control and prevention of PEDV. A PEDV strain obtained from NVSL has been successfully grown in Vero cells. Additionally, a PEDV field strain obtained from a swine diagnostic case submitted to the U of MN VDL has been adapted to grow in cells. Now that PEDV has been successfully isolated in vitro, efforts are underway to develop serological assays (IPMA and IFA) for the detection of PEDV antibodies. PEDV positive intestine and fecal samples have been deposited in the University of Minnesota Infectious Agent Depository, with additional PEDV samples to be deposited in the future. The samples will be available to PEDV researchers for future assay development and research. Complete genome sequences have been obtained from 13 PEDV strains found in the United States swine population. These American PEDV strains were genetically similar, with 99.8 to 100% nucleotide identity. However, phylogenetic analyses indicate the emergence of two distinct American PEDV clades. As PEDV continues to spread throughout the US, PEDV strain variation is expected to increase. A comparison between the U of MN VDL PEDV qRT-PCR and a commercial PEDV detection kit (Life Technology) indicated superior performance by the U of MN VDL qRT-PCR. The commercial kit missed several positive PEDV samples, all of which were confirmed PEDV positive by a secondary U of MN VDL RT-PCR assay targeting the N gene.