#16-060

Complete

Category

Date Full Report Received

06/28/2018

Date Abstract Report Received

06/28/2018

Investigation

Institution:
Primary Investigator:

Influenza A virus of swine (IAV-S) is widespread and causes significant losses to swine producers. Besides its economic burden, the virus also poses a great threat to public health due to its zoonotic potential. Therefore, successful control of IAV-S will not only reduce the economic impact of this viral pathogen to the swine industry but also alleviate the threat to public health. The substantial viral genomic diversity is the main challenge for the development of a broadly protective vaccine against IAV-S. One effective approach to overcome the extraordinary genetic diversity of RNA viruses is to computationally design a consensus vaccine immunogen based on a large number of field virus sequences. It has been demonstrated in the case of several important RNA viruses such as human immunodeficiency virus (HIV), human influenza virus and porcine reproductive and respiratory syndrome viruses (PRRSV) that vaccines based on consensus sequences elicit broader levels of cross-reactive immune responses than vaccine based on naturally occurring sequences. In this project, we sought to determine if a consensus hemagglutinin (HA) protein would elicit a broader level of protection than a naturally occurring HA protein. To address this question, we computationally designed a consensus hemagglutinin subtype 3 (H3) vaccine antigen based on a set of 1,112 H3 sequences of IAV-S deposited on GenBank from 2011 to 2015. Pigs vaccinated with the consensus H3 antigen elicited a broad spectrum of neutralizing antibodies. Importantly, these vaccinated pigs were protected against challenge with a heterologous H3 IAV-S strain. Collectively, our data provide a proof-of-evidence that the consensus immunogen approach can be employed to develop a broadly protective vaccine against IAV-S.