PEDV – Feed

Feasibility of viability PCR and ex-vivo bioassay to detect viable PED virus in feed

Objectives of Research Project:

1. To evaluate the feasibility of viability-PCR (v-PCR) to identify viable PEDV in feed samples.

Post-processing contamination chemical mitigation strategies to control PEDV in feed and feed ingredients

Carthage Innovative Swine Solutions, LLC; Greiner

Objectives of Research Project:

Objectives of Research Project:

Objective 1: Confirm the minimum infectious dose of PEDV in a feed matrix using a 10 d old pig bioassay.

Objective 2: Determine the impact of conditioning time and temperature of pelleted complete feed at 2 PEDV dosages on PEDV inactivation as measured by RT-PCR and bioassay.

Objectives of Research Project:

There have been multiple recent outbreaks of porcine epidemic diarrhea virus (PEDV) in isolated, high health nurseries following the migration of red-winged blackbirds that coincided with feed deliveries (http://www.cvm.umn.edu/sdec/prod/groups/cvm/@pub/@cvm/@sdec/documents/content/cvm_content_475778.pdf). In these outbreaks it was noted that bin tops were coated in bird feces that was subsequently found to be PEDV PCR-positive. It seems, then, that birds may serve as a potential and ever-present source of feed contamination at swine farms. The objective of this survey is to characterize whatrole Sturnus vulgaris(the Common Starling) may serve as a potential vector for PEDV and swine deltacoronavirus (SdCV). For each virus, the investigators wish to determine:

1. Can starlings serve as a mechanical vector
2. Can starlings pass virus in their feces
3. Can virus replicate within the intestines of starlings