PEDV 2013 Research

To assist producers and their veterinarians in the management, control and potential elimination of the virus, the National Pork Board funded key research projects to better understand PEDV. In order to provide timely information to producers from those projects, the objectives and initial updates will be periodically reported.
NOTE: The updates from the proposals represent interim information only and are not intended to be final reports. The final and formal reports will be provided at the end of the terms of the projects and then posted online at pork.org. The information in these updates is intended to inform stakeholders of progress, but are not intended to be the final outcome or recommendation.

For further information, please contact Dr. Lisa Becton, Checkoff’s Director of Swine Health Information and Research, at LBecton@pork.org.

Evaluation of Stalosan F disinfectant to inactivate Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Virus when applied to commercial hog trailers

NPB# 13-248 Iowa State University

Objectives:
The objective of this study was to investigate the efficacy of using Stalosan F disinfectant to inactivate PEDv and PRRSv in swine feces on metal surfaces similar to what is found in livestock trailers after fecal and other organic matter has been removed by scraping and sweeping, but not washed.

Environmental Stability of PEDV

NPB# 13-215 University of Minnesota

Objectives:
Determine the environmental stability of PED (porcine epidemic diarrhea virus):

  • Aim 1. To determine survival of PED in fresh feces that represents the risk posed by transport.
  • Aim 2: To determine survival of PED in slurry (old feces in the pit) that reflects the risk of manure spreading.
  • Aim 3. To study PED survival in drinking and recycled water (truck washes).
  • Aim 4. To study PED survival in animal feed.

Epidemiologic investigation on propensity for lateral spread of PEDV

NPB# 13-216 University of Minnesota

Objectives:

  1. Prevalence & incidence of PED virus contamination in transport vehicles at slaughter.
  2. Determine incidence of infection in unrelated sites adjacent to known infected sites and identify site-level risk factors associated with infection

Propagation of PEDV in tissue culture and development of standardized reference samples for use in diagnostic testing

NPB# 13-22 The Ohio State University

Objectives:

  1. Development and validation of diagnostic testing for PEDV antigen and antibody detection
  2. Development of standardized reference samples that can be utilized by Veterinary Diagnostic Labs (VDLs) for diagnostic test validation
  3. Develop and validate antibody-based diagnostic tests for serologic monitoring and surveillance

Oral fluid testing for cost-effective and efficient surveillance and control of PEDV in swine population

NPB# 13-226 Iowa State University

Objectives:

  1. Determine if oral fluids can be sample matrix to detect PEDv and/or virus-specific antibody.
  2. Evaluate the performance of PEDv diagnostics on oral fluid samples.
  3. Assess the utility of oral fluid sampling and testing for PEDv monitoring on farm.

Evaluation of time and temperature sufficient to kill PEDV in swine feces on metal surfaces

NPB# 13-227 Iowa State University

Objective:
The objective of this study is to investigate the combinations of time and temperature sufficient to kill porcine epidemic diarrhea virus (PEDV) in swine feces on the metal surface of a model trailer used to haul live pigs. The results of this study will inform producers and veterinarians about the adequacy of current sanitation procedures for live haul trailers and enable them to make better decisions about investments in truck sanitation.

Tissue localization, shedding, virus carriage, antibody response, and aerosol transmission of PEDV following inoculation of feeder pigs

NPB# 13-228 Kansas State University

Objective:
Study of the basic pathogenesis and characterization of the virus: Tissue localization, shedding, virus carriage, antibody response, and aerosol transmission of Porcine Epidemic Diarrhea Virus (PEDV) following inoculation of feeder pigs will be investigated. In an attempt to expand diagnostic testing capabilities, multiple aliquots of all will be samples collected and shared with requesting laboratories

Development and validation of isolation and diagnostic testing detection for PEDV

NPB# 13-238 University of Minnesota

Objectives:

  1. Development and standardization of viral propagation techniques to produce virus for use in diagnostic testing (outcome to be publicly available to all VDLs for PEDv diagnostics).
  2. Development of standardized reference samples that can be utilized by VDLs for diagnostic test validation.
  3. Validation of the current PCR diagnostic tests.

Development and validation for diagnostic testing for antigen and antibody detection for PEDV

NPB# 13-239 University of Minnesota

Objectives:

  1. Develop and validate antibody-based diagnostic tests for serologic monitoring and surveillance.
  2. Develop and validate oral fluid testing (antigen and antibody testing capabilities) to provide rapid method for sample collection and to support on-farm surveillance.

 

Defining PEDV maternal humoral immunity and correlates of neonatal protection

NPB# 13-264 Iowa State University

Objectives:

  1. Evaluate PEDV protection in piglets from immune sows.  Assess piglet protection against PEDV challenge in the context of maternal immunity against PEDV.
  2. Evaluate PEDV protection in naïve piglets administered specific levels of anti-PEDV antibody.  Assess piglet protection against PEDV challenge in the context of specific levels of passively acquired anti-PEDV antibody.